Tag
Part:BBa_K3093012:Design
Designed by: Liu Xu Group: iGEM19_ECUST_China (2019-10-16)
HlyA-tag (OmpT-cleavable linker)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
F-primer: ggtagcgcacgacgagcaggcggtggcggttctttagcctatggaagtcaggg R-primer :cgcctgctcgtcgtgcgctaccgccaccgccagcaccggtggagtgacgac
Source
References
[1]Kramer RA, Zandwijken D, Egmond MR, Dekker N. In vitro folding, purification and characterization of Escherichia coli outer membrane protease ompT. Eur J Biochem. 2000 Feb;267(3):885-93. PubMed PMID: 10651827.